Potential of Time-Resolved Serial Femtosecond Crystallography Using High Repetition Rate XFEL Sources

This perspective review describes emerging techniques and future opportunities for time-resolved serial femtosecond crystallography (TR-SFX) experiments using high repetition rate XFEL sources. High sources are becoming more available with the European in operation recently upgraded LCLS-II will be near future. One efficient use of these facilities TR-SFX relies on pump–probe a laser to trigger reaction light-responsive proteins or mix-and-inject light-unresponsive proteins. With view widen application TR-SFX, promising field photocaged compounds is under development, which allows very fast triggering reactions that no longer limited naturally samples. In addition triggering, key concern when performing an SFX experiment sample usage, main focus new rate-compatible delivery methods.